Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Translational Control

TIAR (D32D3) XP® Rabbit mAb #8509

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H M R Mk Endogenous 42 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TIAR (D32D3) XP® Rabbit mAb recognizes endogenous levels of total TIAR protein. This antibody also weakly recognizes recombinant TIA1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIAR protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TIAR (D32D3) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using TIAR (D32D3) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using TIAR (D32D3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

TIAR is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins (1,2). It functions as a translational repressor under conditions of cellular damage (3,4). In response to cellular stress, TIAR associates with eIF1, eIF3, and the 40S ribosomal subunit and forms noncanonical preinitiation complexes that are translationally inactive (3,4). TIAR then aggregates with its family member TIA1 and facilitates the accumulation of the translationally inactive preinitiation complexes into discrete cytoplasmic foci called stress granules. The two major isoforms of TIAR are the products of alternative mRNA splicing (5,6).

  1. Tian, Q. et al. (1991) Cell 67, 629-39.
  2. Kawakami, A. et al. (1992) Proc Natl Acad Sci USA 89, 8681-5.
  3. Anderson, P. and Kedersha, N. (2002) Cell Stress Chaperones 7, 213-21.
  4. Anderson, P. and Kedersha, N. (2002) J Cell Sci 115, 3227-34.
  5. Kawakami, A. et al. (1994) J Immunol 152, 4937-45.
  6. Beck, A.R. et al. (1996) Nucleic Acids Res 24, 3829-35.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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