Product Pathways - Apoptosis
Caspase-14 Antibody #8519
PhosphoSitePlus® protein, site, and accession data: Casp14
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R (Mk) | Endogenous | 28-30, 11 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 8519:
- Western Blotting
Specificity / Sensitivity
Caspase-14 Antibody recognizes endogenous levels of total caspase-14 protein and the p11 subunit of mature caspase-14.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human caspase-14 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from mouse and rat skin using Caspase-14 Antibody.
Western Blotting
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with an expression construct encoding human caspase-14 (hCaspase-14, +), using Caspase-14 Antibody.
Background
Caspases are a family of cysteine proteases that play an essential role in carrying out apoptosis. Caspase-14, also named MICE, is a unique member of the caspase family with restricted expression; it is found in embryonic tissues and adult skin (1,2). Caspase-14 is weakly processed into p18 and p11 subunits by caspase-8 (2). Caspase-14 may not play a role in apoptosis (2), but instead may regulate keratinocyte differentiation (3). Expression of caspase-14 may protect from psoriasis (4,5) and irradiation damage (6). Caspase-14 may also be responsible for proteolytic processing of filaggrin during terminal differentiation of keratinocytes (6).
- Hu, S. et al. (1998) J Biol Chem 273, 29648-53.
- Van de Craen, M. et al. (1998) Cell Death Differ 5, 838-46.
- Eckhart, L. et al. (2000) Biochem Biophys Res Commun 277, 655-9.
- Lippens, S. et al. (2004) Am J Pathol 165, 833-41.
- Walsh, D.S. et al. (2005) J Dermatol Sci 37, 61-3.
- Denecker, G. et al. (2007) Nat Cell Biol 9, 666-74.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
