Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Cytoskeletal Signaling

Caveolin-2 (D4A6) XP® Rabbit mAb #8522

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H Mk Endogenous 21 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Caveolin-2 (D4A6) XP® Rabbit mAb recognizes endogenous levels of total caveolin-2 protein. Based on amino acid sequence, the antibody is not expected to cross-react with caveolin-1 or caveolin-3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human caveolin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Caveolin-2 (D4A6) XP® Rabbit mAb.

IP

IP

Immunoprecipitation of caveolin-2 from HeLa cell extracts using Caveolin-2 (D4A6) XP® Rabbit mAb. Western blot was performed using the same antibody. Lane 1 is 10% input.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Caveolin-2 (D4A6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).

  1. Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
  2. Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304.
  3. Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
  4. Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
  5. Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75.
  6. Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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