Cell Signaling Technology

Product Pathways - Development

TEAD1 Antibody #8526

Applications Reactivity Sensitivity MW (kDa) Source
W H R Mk (M) Endogenous 50 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TEAD1 Antibody recognizes endogenous levels of total TEAD1 protein. This antibody does not cross-react with other TEAD proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TEAD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from ACHN and A549 cells using TEAD1 Antibody.

Background

The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of Yes-associated protein (YAP), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). When the Hippo pathway is turned off, YAP is phosphorylated and translocates to the nucleus where it associates with various transcription factors including members of the transcriptional enhancer factor (TEF) family, also known as the TEA domain (TEAD) family (TEAD1-4) (5,6). Although widely expressed in tissues, the TEAD family proteins have specific tissue and developmental distributions. YAP/TEAD complexes regulate the expression of genes involved in cell proliferation and apoptosis (5).

  1. Pan, D. (2010) Dev Cell 19, 491-505.
  2. Harvey, K.F. et al. (2003) Cell 114, 457-67.
  3. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
  4. Zhao, B. et al. (2008) Curr Opin Cell Biol 20, 638-46.
  5. Zhao, B. et al. (2008) Genes Dev 22, 1962-71.
  6. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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