Cell Signaling Technology

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Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) #8527

Applications Reactivity Sensitivity Isotype
IF-IC Endogenous Mouse IgG1

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) is not directed against any known antigen. It functions as an isotype control for mouse IgG1 monoclonal antibodies.

Source / Purification

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 594 Conjugate) #7319 (left) compared to Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) (right) at matched concentration. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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