Cell Signaling Technology

Product Pathways - Metabolism

ATPIF1 Antibody #8528

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 12 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ATPIF1 Antibody recognizes endogenous levels of total ATPIF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala37 of human ATPIF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ATPIF1 Antibody.

Background

The ATPase inhibitor factor 1 (ATPIF1) gene encodes a mitochondrial ATPase inhibitor that limits ATP depletion when mitochondrial respiration is impaired (1). ATPIF1 becomes activated following a drop in pH, binding to β-F1-ATPase, thereby inhibiting the hydrolase activity of the H+-ATP synthase (1,2). In addition to its role as an ATP hydrolase, ATPIF1 has also been shown to play a regulatory role in cellular energy metabolism by triggering the induction of aerobic glycolysis in cancer cells resulting in their Warburg phenotype (3,4). The overexpression of ATPIF1 in several human carcinomas further supports its participation in oncogenesis and provides insight into the altered metabolism of cancer cells, which includes the reprogramming of energetic metabolism toward glycolysis (3).

  1. Gledhill, J.R. et al. (2007) Proc Natl Acad Sci USA 104, 15671-6.
  2. Cabezón, E. et al. (2003) Nat Struct Biol 10, 744-50.
  3. Sánchez-Cenizo, L. et al. (2010) J Biol Chem 285, 25308-13.
  4. Wallace, D.C. (2005) Cold Spring Harb Symp Quant Biol 70, 363-74.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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