Cell Signaling Technology

Product Pathways - Translational Control

hnRNP E1 Antibody #8534

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 40 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

hnRNP E1 Antibody recognizes endogenous levels of total hnRNP E1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys237 of human hnRNP E1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using hnRNP E1 Antibody.

Background

hnRNP E1 is a member of the hnRNP family of proteins that are involved in pre-mRNA processing and mRNA export, localization, stability, and translation (1-6). hnRNP E1 exerts a wide range of biological functions, such as transcriptional activation of mouse MOR gene expression (7), attenuation of alternative splicing of GHR pseudoexon expression (8), stabilization of collagen I and II (9), beta-globin (10), and androgen receptor (11) mRNAs, and regulation of translation of various genes including Dab2, ILEI, and Bag-1 (12,13). hnRNP E1 is ubiquitously expressed. Phosphorylation of hnRNP E1 affects its RNA binding affinity (13,14).

  1. Cáceres, J.F. et al. (1994) Science 265, 1706-9.
  2. Gallouzi, I.E. and Steitz, J.A. (2001) Science 294, 1895-901.
  3. Carson, J.H. et al. (2001) Results Probl Cell Differ 34, 69-81.
  4. Xu, K. et al. (2001) J Immunol 166, 6358-66.
  5. Habelhah, H. et al. (2001) Nat Cell Biol 3, 325-30.
  6. Dreyfuss, G. et al. (2002) Nat Rev Mol Cell Biol 3, 195-205.
  7. Kim, S.S. et al. (2005) Mol Pharmacol 68, 729-36.
  8. Akker, S.A. et al. (2007) Mol Endocrinol 21, 2529-40.
  9. Thiele, B.J. et al. (2004) Circ Res 95, 1058-66.
  10. Yu, J. and Russell, J.E. (2001) Mol Cell Biol 21, 5879-88.
  11. Yeap, B.B. et al. (2002) J Biol Chem 277, 27183-92.
  12. Pickering, B.M. et al. (2003) Nucleic Acids Res 31, 639-46.
  13. Chaudhury, A. et al. (2010) Nat Cell Biol 12, 286-93.
  14. Leffers, H. et al. (1995) Eur J Biochem 230, 447-53.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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