Cell Signaling Technology

Product Pathways - MAPK Signaling

MKK3 (D4C3) Rabbit mAb #8535

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 38-40 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MKK3 (D4C3) Rabbit mAb recognizes endogenous levels of total MKK3 protein. This antibody cross-reacts slightly with MKK6 but demonstrates an approximate 10-fold preference for MKK3 using recombinant, purified proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MKK3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MKK3 (D4C3) Rabbit mAb.

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5).

  1. Derijard, B. et al. (1995) Science 267, 682-685.
  2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
  4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
  5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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