Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Rad17 (D3G6) Rabbit mAb #8561

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Endogenous 80 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rad17 (D3G6) Rabbit mAb recognizes endogenous levels of total Rad17 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rad17 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rad17 (D3G6) Rabbit mAb.

Background

The human checkpoint protein Rad17 and its fission and budding yeast orthologues (Schizosaccharomyces pombe Rad17 and Saccharomyces cerevisiae Rad24, respectively) are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR-dependent phosphorylation of Rad17 at Ser635 and Ser645. Rad17 phosphorylation is a critical early event during checkpoint signaling in DNA-damaged cells (5-7).

  1. Griffiths, D. J. et al. (1995) EMBO J. 14, 5812-5823.
  2. Li, L. et al. (1999) Oncogene 18, 1689-1699.
  3. Bao, S. et al. (1998) Cell Growth Differ. 9, 961-967.
  4. von Deimling, F. et al. (1999) Hum. Genet. 105, 17-27.
  5. Bao, S. et al. (2001) Nature 411, 969-973.
  6. Post, S. et al. (2001) PNAS 98, 13102-13107.
  7. Wang, X. et al. (2001) Cancer Research 61, 7417-7421.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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