Product Pathways - Metabolism
PCK2 (D3E11) Rabbit mAb #8565
PhosphoSitePlus® protein, site, and accession data: PCK2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H Mk | Endogenous | 71 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
PCK2 (D3E11) Rabbit mAb recognizes endogenous levels of total PCK2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PCK2 protein.
Western Blotting
Western blot analysis of extracts from various cell lines using PCK2 (D3E11) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells uisng PCK2 (D3E11) Rabbit mAb (green) showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
PCK2 (PEPCK2 or PEPCK-M) encodes an isoform of phosphoenolpyruvate carboxykinase (PEPCK) that is found in the mitochondria of renal and hepatic tissues (1). PEPCK is involved in gluconeogenesis, the process of generating glucose from non-carbohydrate substrates such as lactate and glycerol (2). PEPCK catalyzes the reaction whereby oxaloacetate becomes phosphoenolpyruvate, a rate limiting step in the metabolic process (2). The predominant function of PCK2 is to process the lactate that is continually produced by red blood cells in the liver and kidney (1).
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
