Cell Signaling Technology

Product Pathways - Apoptosis

WIPI2 Antibody #8567

Applications Reactivity Sensitivity MW (kDa) Source
W H (Mk) Endogenous 49 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

WIPI2 Antibody recognizes endogenous levels of total human WIPI2 protein. This antibody detects overexpressed mouse WIPI2 protein and weakly detects endogenous levels of mouse WIPI2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu379 of human WIPI2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using WIPI2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full length mouse WIPI2 (mWIPI2; +), using WIPI2 Antibody.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). It is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes.Vacuolar trafficking and autophagy are controlled by the class III type phosphoinositide 3-kinase (PI3K) Vps34, which generates phosphoinositide-3-phosphate (PtdIns3P) (4,5). Atg18 and Atg21 are two related WD-repeat proteins that bind PtdIns3P via a conserved Phe-Arg-Arg-Gly motif (6,7). It has been shown that Atg18 binds to Atg2 and that this complex is directed to vacuolar membranes by its interaction with PtdIns3P (8). Human orthologues of Atg18 and Atg21 were identified as members of the WD-repeat protein Interacting with PhosphoInositides (WIPI) family (9-11). WIPI1 (also called WIPI49) and WIPI2 have been shown to translocate from several vacuolar compartments to LC3-positive autophagosomes during autophagy; this translocation may be used as an autophagy marker (12).

  1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
  2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
  3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
  4. Corvera, S. (2001) Traffic 2, 859-66.
  5. Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.
  6. Krick, R. et al. (2006) FEBS Lett 580, 4632-8.
  7. Strømhaug, P.E. et al. (2004) Mol Biol Cell 15, 3553-66.
  8. Obara, K. et al. (2008) J Biol Chem 283, 23972-80.
  9. Jeffries, T.R. et al. (2004) Mol Biol Cell 15, 2652-63.
  10. Proikas-Cezanne, T. et al. (2007) FEBS Lett 581, 3396-404.
  11. Polson, H.E. et al. (2010) Autophagy 6, Epub ahead of print.
  12. Proikas-Cezanne, T. et al. (2007) FEBS Lett 581, 3396-404.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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