Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

GOPC (D10A12) Rabbit mAb #8576

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 59 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GOPC (D10A12) Rabbit mAb detects endogeneous levels of total GOPC protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe31 of human GOPC protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GOPC (D10A12) Rabbit mAb.

Background

GOPC (FIG) was originally identified as a Golgi-associated, PDZ domain containing protein. It has two coiled-coil domains (CC1 and CC2) located in the amino-terminal region and a PDZ domain in the carboxy-terminal region (1). The CC2 domain and its adjacent linker region mediate the association of GOPC with the golgi protein golgin-160 and the Q-SNARE protein syntaxin 6 (1,2). The PDZ domain of GOPC interacts with the carboxy terminus of target proteins to mediate target protein vesicular trafficking and surface expression (3-6). Fusion of the corresponding GOPC gene with the ROS tyrosine kinase oncogene has been detected in some glioblastomas. The resulting GOPC-ROS fusion protein is targeted to the golgi apparatus where a constitutively activate ROS tyrosine kinase can mediate tumor formation (7,8).

  1. Charest, A. et al. (2001) J Biol Chem 276, 29456-65.
  2. Hicks, S.W. and Machamer, C.E. (2005) J Biol Chem 280, 28944-51.
  3. Cheng, J. et al. (2002) J Biol Chem 277, 3520-9.
  4. He, J. et al. (2004) J Biol Chem 279, 50190-6.
  5. Wente, W. et al. (2005) J Biol Chem 280, 32419-25.
  6. Ito, H. et al. (2006) Biochem J 397, 389-98.
  7. Charest, A. et al. (2003) Proc Natl Acad Sci USA 100, 916-21.
  8. Charest, A. et al. (2006) Cancer Res 66, 7473-81.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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