Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) #8580

Applications Reactivity Sensitivity Isotype
IF-IC F H M R Mk B Pg Endogenous Mouse IgG2a

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total PCNA protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) and propidium iodide (DNA content).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NCI-H460 cells using PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) (green), p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate) #8587 (blue pseudocolor), and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475 (red).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PCNA (PC10) Mouse mAb #2586.

Background

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.

  1. Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res. 23, 3613-3620.
  2. Krishna, T. S. et al. (1994) Cell 79, 1233-1243.
  3. Maga, G. and Hubscher, U. (2003) J. Cell Sci. 116, 3051-3060.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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