Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Dab2 Antibody #8582

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 96 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Dab2 Antibody recognizes endogenous levels of total Dab2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro222 of human Dab2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A172 and A-204 cells using Dab2 Antibody.

Background

Dab2 (disabled homologue 2) is a mitogen responsive phosphoprotein. It exerts multiple functions through association with numerous proteins. Dab2 modulates various signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4), and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and Myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Dab2 is down-regulated in a number of cancers, thereby implicating its role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18).

  1. Hocevar, B.A. et al. (2001) EMBO J 20, 2789-801.
  2. Hocevar, B.A. et al. (2005) J Biol Chem 280, 25920-7.
  3. Hocevar, B.A. et al. (2003) EMBO J 22, 3084-94.
  4. Xu, X.X. et al. (1998) Oncogene 16, 1561-9.
  5. Zhou, J. et al. (2003) J Biol Chem 278, 6936-41.
  6. Mishra, S.K. et al. (2002) EMBO J 21, 4915-26.
  7. Morris, S.M. and Cooper, J.A. (2001) Traffic 2, 111-23.
  8. Keyel, P.A. et al. (2006) Mol Biol Cell 17, 4300-17.
  9. Maurer, M.E. and Cooper, J.A. (2006) J Cell Sci 119, 4235-46.
  10. Maurer, M.E. and Cooper, J.A. (2005) J Cell Sci 118, 5345-55.
  11. Morris, S.M. et al. (2002) Traffic 3, 331-41.
  12. Hasson, T. (2003) J Cell Sci 116, 3453-61.
  13. Huang, C.L. et al. (2006) J Cell Sci 119, 4420-30.
  14. Teckchandani, A. et al. (2009) J Cell Biol 186, 99-111.
  15. Mok, S.C. et al. (1998) Oncogene 16, 2381-7.
  16. Anupam, K. et al. (2006) World J Gastroenterol 12, 6041-5.
  17. Bagadi, S.A. et al. (2007) Breast Cancer Res Treat 104, 277-86.
  18. Chetrit, D. et al. (2011) J Biol Chem 286, 5392-403.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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