Cell Signaling Technology

Product Pathways - NF-kB Signaling

NLRX1 Antibody #8583

Applications Reactivity Sensitivity MW (kDa) Source
W IP H (Mk) Endogenous 100 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NLRX1 Antibody recognizes endogenous levels of total NLRX1 protein. This antibody cross-reacts with a 65 kDa protein of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys441 of human NLRX1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NLRX1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a cDNA construct expressing full-length human NLRX1 (hNLRX1; +), using NLRX1 Antibody.

Background

The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NOD and carboxy-terminal leucine-rich-repeat (LRR) domain involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7).

NLRX1 (CLR11.3/NOD26/NOD5/NOD9) is unique among NLR family members in that it contains an amino-terminal mitochondrial targeting sequence resulting in localization to the mitochondria (8,9). In contrast to most NLR proteins, NLRX1 has been shown to act as a negative regulator of innate immune responses through inhibition of MAVS-Rig-I signaling, as well as inhibition of Toll-like receptor (TLR)-mediated NF-κB activation (9-11). In addition, overexpression of NLRX1 enhanced the production of reactive oxygen species (ROS), resulting in prolonged NF-κB and JNK signaling in response to TNF-α (8).

  1. Elinav, E. et al. (2011) Immunity 34, 665-79.
  2. Inohara, N. et al. (1999) J Biol Chem 274, 14560-7.
  3. Ogura, Y. et al. (2001) J Biol Chem 276, 4812-8.
  4. Sabbah, A. et al. (2009) Nat Immunol 10, 1073-80.
  5. Mariathasan, S. et al. (2004) Nature 430, 213-8.
  6. Agostini, L. et al. (2004) Immunity 20, 319-25.
  7. Martinon, F. et al. (2002) Mol Cell 10, 417-26.
  8. Tattoli, I. et al. (2008) EMBO Rep 9, 293-300.
  9. Moore, C.B. et al. (2008) Nature 451, 573-7.
  10. Allen, I.C. et al. (2011) Immunity 34, 854-65.
  11. Xia, X. et al. (2011) Immunity 34, 843-53.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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