Product Pathways - Neuroscience
Dopamine β-Hydroxylase (DBH) Antibody #8586
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Dopamine β-Hydroxylase (DBH) Antibody recognizes endogenous levels of total DBH protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala211 of human dopamine β-hydroxylase protein. Antibodies are purified by protein A and peptide affinity chromatography.
Dopamine β-Hydroxylase (DBH) is an enzyme of the copper type II ascorbate-dependent mono-oxygenase family. This enzyme forms homotetramers composed of two noncovalently bound disulfide-linked dimers and is found as both membrane-associated and soluble forms (1-3). The soluble form is present in the lumen of secretory granules (4) and is released from cells by exocytosis (5). DBH converts dopamine to noradrenaline (6). Deficiency in this enzyme causes a rare disease characterized by a complete absence of noradrenaline and adrenaline in plasma together with increased plasma dopamine levels (7). Orthostatic hypotension, the main symptom of DBH deficiency, can be alleviated by administration of dihydroxyphenylserine, a synthetic precursor of noradrenaline (8).
- Smith, W.J. and Kirshner, N. (1967) Mol Pharmacol 3, 52-62.
- Lagercrantz, H. (1976) Neuroscience 1, 81-92.
- Winkler, H. (1976) Neuroscience 1, 65-80.
- Laduron, P.M. (1975) FEBS Lett 52, 132-4.
- Weinshilboum, R.M. et al. (1971) Science 174, 1349-51.
- Kaufman, S. and Friedman, S. (1965) Pharmacol Rev 17, 71-100.
- Robertson, D. et al. (1986) N Engl J Med 314, 1494-7.
- Biaggioni, I. and Robertson, D. (1987) Lancet 2, 1170-2.
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