Product Pathways - Neuroscience
Dopamine β-Hydroxylase (DBH) Antibody #8586
PhosphoSitePlus® protein, site, and accession data: DBH
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 75-80 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 8586:
- Western Blotting
Specificity / Sensitivity
Dopamine β-Hydroxylase (DBH) Antibody recognizes endogenous levels of total DBH protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala211 of human dopamine β-hydroxylase protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from SH-SY5Y cells, untreated (-) or treated with TPA (200 nM, 24 hr) #4174 (+), and 293 cells, using Dopamine β-Hydroxylase (DBH) Antibody.
Background
Dopamine β-Hydroxylase (DBH) is an enzyme of the copper type II ascorbate-dependent mono-oxygenase family. This enzyme forms homotetramers composed of two noncovalently bound disulfide-linked dimers and is found as both membrane-associated and soluble forms (1-3). The soluble form is present in the lumen of secretory granules (4) and is released from cells by exocytosis (5). DBH converts dopamine to noradrenaline (6). Deficiency in this enzyme causes a rare disease characterized by a complete absence of noradrenaline and adrenaline in plasma together with increased plasma dopamine levels (7). Orthostatic hypotension, the main symptom of DBH deficiency, can be alleviated by administration of dihydroxyphenylserine, a synthetic precursor of noradrenaline (8).
- Smith, W.J. and Kirshner, N. (1967) Mol Pharmacol 3, 52-62.
- Lagercrantz, H. (1976) Neuroscience 1, 81-92.
- Winkler, H. (1976) Neuroscience 1, 65-80.
- Laduron, P.M. (1975) FEBS Lett 52, 132-4.
- Weinshilboum, R.M. et al. (1971) Science 174, 1349-51.
- Kaufman, S. and Friedman, S. (1965) Pharmacol Rev 17, 71-100.
- Robertson, D. et al. (1986) N Engl J Med 314, 1494-7.
- Biaggioni, I. and Robertson, D. (1987) Lancet 2, 1170-2.
Application References
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Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 4174 TPA (12-O-Tetradecanoylphorbol-13-Acetate)
For Research Use Only. Not For Use In Diagnostic Procedures.
