Product Pathways - Neuroscience
APBA2 Antibody #8593
PhosphoSitePlus® protein, site, and accession data: APBA2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | M R (H) | Endogenous | 135 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
APBA2 Antibody recognizes endogenous levels of total APBA2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe300 of human APBA2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from mouse and rat brain using APBA2 Antibody.
Western Blotting
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human APBA2 (hAPBA2, +), using APBA2 Antibody.
Background
Amyloid β A4 precursor protein-binding family A member 2 (APBA2) is a neuronal adaptor protein that interacts with the amyloid β precursor protein (APP) (1). The amyloid β-protein (Aβ) is the principal component of amyloid plaques, a pathological hallmark of Alzheimer’s disease (2). APBA2 has been shown to stabilize APP metabolism and suppress the secretion of Aβ (3). APBA2 mediates interaction between APP and the neural type I membrane protein Alcadein (Alc) by linking their cytoplasmic domains, thereby forming a tripartite complex of the three proteins in neurons (4).
- Sakuma, M. et al. (2009) J Neurochem 109, 465-75.
- Saito, Y. et al. (2008) J Biol Chem 283, 35763-71.
- Araki, Y. et al. (2003) J Biol Chem 278, 49448-58.
- Sano, Y. et al. (2006) J Biol Chem 281, 37853-60.
Application References
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- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.
