Cell Signaling Technology

Product Pathways - Neuroscience

NHERF1 (D1C10) Rabbit mAb #8601

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 50 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NHERF1 (D1C10) Rabbit mAb recognizes endogenous levels of total NHERF1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala140 of human NHERF1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from T-47D and MCF7 cells using NHERF1 (D1C10) Rabbit mAb.

Background

Na+/H+ exchanger regulatory factor (NHERF1 or EBP-50) is one of several related PDZ domain-containing proteins (1). NHERF1 was first identified as a necessary cofactor for cyclic AMP-associated inhibition of Na+/ H+ exchanger isoform 3 (NHE3) (2). NHERF1 is a multifunctional adaptor protein that interacts with receptors and ion transporters via its PDZ domains, and with the ERM family of proteins, including merlin, via its carboxy-terminus (2,3). NHERF1 may play an important role in breast cancer. Estrogen has been found to induce NHERF1 in estrogen receptor-positive breast cancer cells (2,3). Furthermore, NHERF1 has been shown to bind to PDGFR, which is activated in breast carcinomas. NHERF1 has been found to promote the formation of a ternary complex containing PTEN, NHERF1, and PDGFR. Therefore, NHERF1 may function to recruit PTEN to PDGFR to inhibit the activation of PI3K/Akt signaling in normal cells; this mechanism may be disrupted in cancer (4). NHERF1 also binds to the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as an ion channel and has disease-causing mutations in cystic fibrosis (5). Other proposed functions of NHERF1 include testicular differentiation, endosomal recycling, membrane targeting, protein sorting, and trafficking (6).

  1. Donowitz, M. et al. (2005) J Physiol 567, 3-11.
  2. Voltz, J.W. et al. (2001) Oncogene 20, 6309-14.
  3. Stemmer-Rachamimov, A.O. et al. (2001) Am J Pathol 158, 57-62.
  4. Takahashi, Y. et al. (2006) EMBO J 25, 910-20.
  5. Wheeler, D. et al. (2007) J Biol Chem 282, 25076-87.
  6. Weinman, E.J. et al. (2000) Am J Physiol Renal Physiol 279, F393-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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