Cell Signaling Technology

Product Pathways - Metabolism

MDH2 Antibody #8610

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 35 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MDH2 Antibody recognizes endogenous levels of total MDH2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MDH2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MDH2 Antibody.

Background

Malate dehydrogenase (MDH) is a key enzyme in the tricarboxylic acid cycle and malate/aspartate shuttle (1,2). MDH is widely expressed in organisms from most bacteria to all eukaryotes (2). The cytoplasmic MDH isoenzyme (cMDH or MDH1) primarily reduces oxaloacetate to malate in the malate/aspartate shuttle (1-3). The major function of the mitochondrial MDH isoenzyme (mMDH or MDH2) is to oxidize malate to oxaloacetate in the tricarboxylic acid cycle (1,2).

  1. Matsuda, T. et al. (2010) J Pharmacol Sci 112, 320-6.
  2. Minárik, P. et al. (2002) Gen Physiol Biophys 21, 257-65.
  3. Joh, T. et al. (1987) J Biol Chem 262, 15127-31.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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