Cell Signaling Technology

Product Pathways - Metabolism

CA2 Antibody #8612

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 28 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CA2 Antibody recognizes endogenous levels of total CA2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys169 of human CA2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and MCF7 cells using CA2 Antibody.

Background

Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (α, β, and γ) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the α class (1,2).

CA2 is a cytosolic member of the α class. It is the most widely distributed isoform among the mammalian CAs (1). Defects in CA2 are associated with osteopetrosis and renal tubular acidosis (3-5). Elevated expression of CA2 is observed in patients with Alzheimer’s disease and the developing brains of Down syndrome patients (6,7). CA2 is also overexpressed in Gastrointestinal Stromal Tumors (GISTs) and is considered a useful marker for diagnosis (8). Recently, CA2 was reported to facilitate transporter activity of the monocarboxylate transporter isoform 1 and 4 (MCT1/4) independent of its own catalytic activity (9,10)

  1. Smith, K.S. et al. (1999) Proc Natl Acad Sci USA 96, 15184-9.
  2. Tripp, B.C. et al. (2001) J Biol Chem 276, 48615-8.
  3. McMahon, C. et al. (2001) Blood 97, 1947-50.
  4. Borthwick, K.J. et al. (2003) J Med Genet 40, 115-21.
  5. Lai, L.W. et al. (1998) J Clin Invest 101, 1320-5.
  6. Palminiello, S. et al. (2008) Brain Res 1190, 193-205.
  7. Jang, B.G. et al. (2010) J Alzheimers Dis 21, 939-45.
  8. Parkkila, S. et al. (2010) Mod Pathol 23, 743-50.
  9. Becker, H.M. et al. (2005) J Biol Chem 280, 39882-9.
  10. Becker, H.M. et al. (2011) Proc Natl Acad Sci USA 108, 3071-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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