Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Cytoskeletal Marker Antibody Sampler Kit #8614

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
Desmin (D93F5) XP® Rabbit mAb #5332 40 µl W IF-F IF-IC H M R (Mk) 53 Rabbit IgG
Keratin 17 (D73C7) XP® Rabbit mAb #4543 40 µl W IF-IC F H M R (Mk) (Dg) 48 Rabbit IgG
Pan-Keratin (C11) Mouse mAb #4545 40 µl W IHC-P IF-P IF-IC F H R Mk 46-58 Mouse IgG1
β-Tubulin (9F3) Rabbit mAb #2128 40 µl W IHC-P IF-IC F H M R Mk Z B (C) 55 Rabbit IgG
Vimentin (D21H3) XP® Rabbit mAb #5741 40 µl W IHC-P IF-IC F H M R Mk 57 Rabbit IgG
β-Actin (D6A8) Rabbit mAb #8457 40 µl W IF-IC F H M R Mk 45 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl Horse

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-P=Immunofluorescence (Paraffin)  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  Z=Zebrafish  B=Bovine  Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Desmin (D93F5) XP® Rabbit mAb, Keratin 17 (D73C7) XP® Rabbit mAb, β-Tubulin (9F3) Rabbit mAb, and Vimentin (D21H3) XP® Rabbit mAb, and B-Actin (D68) Rabbit mAb recognize endogenous levels of total respective target proteins. Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13, and 18. This antibody does not cross-react with other keratins.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7, NIH/3T3, and PC12 cells using β-Tubulin (9F3) Rabbit mAb #2128.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) XP® Rabbit mAb #4543. As expected, A549 cells are negative for keratin 17.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pan-Keratin (C11) Mouse mAb #4545.


Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 cells, and rat and human heart using Desmin (D93F5) XP® Rabbit mAb #5332.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb #5741.

Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb #8457.


Description

The Cytoskeletal Marker Antibody Sampler Kit provides an economical means to evaluate the presence and status of select cytoskeleton associated proteins. The kit provides enough primary antibodies to perform four western blots per primary antibody.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide cooresponding to residues near the amino terminus of human β-actin protein, carboxy terminal residues of human desmin protein, residues near the carboxy terminus of human keratin 17 protein, the amino terminus of human β-tubulin protein, or residues surrounding Arg45 of human vimentin protein. Mouse monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A-431 cells.

Background

The cytoskeleton consists of three different types of cystosolic fibers: microtubules, microfilaments (actin) and intermediate filaments. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Major types of intermediate filaments are distinguished in part by the tissue in which they are expressed, for example; cytokeratins (epithelial cells), vimentin (mesenchyme origin), and desmin (skeletal, visceral and certain vascular smooth muscle cells) (2). Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form intermediate filaments (3). Research studies have demonstrated that vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers in order to distinguish between the two forms of neoplasm (4). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs (5). α/β-tubulin heterodimers form the tubulin subunit that comprises the microtubule building block (6)..

  1. Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.
  2. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  3. Moll, R. et al. (1982) Cell 31, 11-24.
  4. Leader, M. et al. (1987) Histopathology 11, 63-72.
  5. Li, Z. et al. (1996) Dev Biol 175, 362-6.
  6. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

* Product-specific protocol.

Companion Products

Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc. 

For Research Use Only. Not For Use In Diagnostic Procedures.

Products