Cell Signaling Technology

Product Pathways - Metabolism

Transketolase Antibody #8616

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 68 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Transketolase Antibody recognizes endogenous levels of total transketolase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val296 of human transketolase protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Transketolase Antibody.

Background

Transketolase (TKT) is a homodimer in the pentose phosphate pathway (PPP) that catalyzes the interketol transfer between ketoses and aldoses (1,2). This enzyme, along with transaldolase, connects the nonoxidative branch of the PPP with glycolysis (1-3). Several regions of TKT are evolutionarily conserved from gram-negative bacteria to mammals (3). There is evidence that hypoxic (4) and non-hypoxic induction of HIF1-α (5) increases the expression of TKT. Because cancer cells rely on TKT in altered cell metabolism for nucleic acid synthesis, work has been done to develop inhibitors of TKT as novel cancer treatments (5-8).

  1. Mitschke, L. et al. (2010) J Biol Chem 285, 31559-70.
  2. Esakova, O.A. et al. (2005) Life Sci 78, 8-13.
  3. Schenk, G. et al. (1997) J Mol Evol 44, 552-72.
  4. Haseloff, R.F. et al. (2006) Proteomics 6, 1803-9.
  5. Zhao, F. et al. (2010) Oncogene 29, 2962-72.
  6. Thomas, A.A. et al. (2008) Bioorg Med Chem Lett 18, 2206-10.
  7. Thomas, A.A. et al. (2008) Bioorg Med Chem Lett 18, 509-12.
  8. Raïs, B. et al. (1999) FEBS Lett 456, 113-8.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products