Product Pathways - Neuroscience
Synaptic Vesicle Antibody Sampler Kit #8630
|8630S||1 Kit (4 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Munc18-1 Antibody #8474||40 µl||W||H, M, R||Mk||70||Rabbit|
|Syntaxin 6 (C34B2) Rabbit mAb #2869||40 µl||W, IP, IF-IC||H, M, R||32||Rabbit IgG|
|SNAP25 (D9A12) Rabbit mAb #5309||40 µl||W, IP||H, M, R||25||Rabbit IgG|
|NSF (D31C7) XP® Rabbit mAb #3924||40 µl||W, IP, IF-F||H, M, R, Mk||78||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Western blot analysis of extracts from various cell lines using Syntaxin 6 (C34B2) Rabbit mAb #2869.
Western blot analysis of extracts from various cell lines using NSF (D31C7) XP® Rabbit mAb #3924.
Western blot analysis of extracts from mouse and rat brain using SNAP25 (D9A12) Rabbit mAb #5309.
Western blot analysis of extracts from mouse brain, rat brain, and human cerebellum using Munc18-1 Antibody #8474.
Western blot analysis of extracts from rat brain synaptic fractions using NSF (D31C7) XP® Rabbit mAb #3924, Munc18-1 Antibody #8474, Syntaxin 6 (C34B2) Rabbit mAb #2869, and SNAP25 (D9A12) Rabbit mAb #5309. Equal loading of each fraction was assessed using β-Tubulin (9F3) Rabbit mAb #2128. Separation of the different synaptic fractions was confirmed using PSD95 (D27E11) XP® Rabbit mAb # 3450. Fractionation of the different synaptic compartments was carried out as described by Phillips, G.R. et al. (2001) Neuron 32, 63–77.
The Synaptic Vesicle Antibody Sampler Kit provides an economical means of evaluating proteins involved in synaptic vesicle fusion and membrane trafficking. The kit contains enough primary and secondary antibodies to perform four western miniblot experiments with each antibody.
Specificity / Sensitivity
Munc18-1 Antibody recognizes endogenous levels of total Munc18-1 protein. NSF (D31C7) XP® Rabbit mAb detects endogenous levels of total NSF protein. SNAP25 (D9A12) Rabbit mAb detects endogenous levels of total SNAP25 protein. Syntaxin 6 (C34B2) Rabbit mAb detects endogenous levels of total syntaxin 6 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near Asp453 of human Munc18-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr140 of mouse syntaxin 6 protein, residues surrounding Gln116 of human SNAP25 protein, or residues surrounding Leu524 of human NSF protein.
Fusion of a vesicle to its target membrane is a universal process in eukaryotic cells for proper cellular organization and function. Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca2+-dependent exocytosis (3). Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (4,5). Munc18-1 acts as a molecular chaperone for syntaxin-1, allowing for formation of the SNARE complex at the plasma membrane (6). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (7,8). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion, and eventual recycling of the SNARE complex for subsequent membrane fusion (7,8).
- Ungermann, C. and Langosch, D. (2005) J Cell Sci 118, 3819-28.
- Leabu, M. J Cell Mol Med 10, 423-7.
- Salaün, C. et al. (2004) Biochim Biophys Acta 1693, 81-9.
- Bock, J.B. et al. (2001) Nature 409, 839-41.
- Bock, J.B. et al. (1996) J Biol Chem 271, 17961-5.
- Medine, C.N. et al. (2007) J Cell Sci 120, 4407-15.
- May, A.P. et al. (2001) J Biol Chem 276, 21991-4.
- Dalal, S. et al. (2004) Mol Biol Cell 15, 637-48.
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* Product-specific protocol.
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9998 BSA
- 9999 Nonfat Dry Milk
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 7075 Anti-biotin, HRP-linked Antibody
- 5297 Synapsin-1 (D12G5) XP® Rabbit mAb
- 5308 SNAP25 (D7B4) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.