Product Pathways - Nuclear Receptor Signaling
NRBF-2 (D8G1) Rabbit mAb #8633
PhosphoSitePlus® protein, site, and accession data: NRBF2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk (Hm) (B) (Dg) (Hr) | Endogenous | 40 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
B=Bovine
Dg=Dog
Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
NRBF-2 (D8G1) Rabbit mAb recognizes endogenous levels of total NRBF-2 protein. Based upon sequence alignment, this antibody is predicted to cross-react with NRBF-1/COPR1.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NRBF-2 protein.
Western Blotting
Western blot analysis of extracts from various cell lines using NRBF-2 (D8G1) Rabbit mAb.
Background
Nuclear Receptor Binding Factor-2 (NRBF-2), also referred to as Comodulator of PPAR and RXRα-2 (COPR-2), has been shown to interact with the AF-2 region of several nuclear hormone receptors with varying affinities such as PPARα, RARα, RARγ, and RXRα (1,2). NRBF-2 contains a LLYLL motif, which matches the LXXLL NR box consensus and is required for functional NRBF-2/nuclear receptor complex formation and repression of receptor function. NRBF-2 also contains a unique autonomous activation domain and, thus, does not completely abrogate nuclear receptor function, suggesting that NRBF-2 might serve as a molecular rheostat to fine-tune the transcriptional activity of liganded nuclear receptors (1,2).
- Yasumo, H. et al. (2000) Biochim Biophys Acta 1490, 189-97.
- Flores, A.M. et al. (2004) J Invest Dermatol 123, 1092-101.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 8066 NRBF-2 Antibody
- 5948 NCoR1 Antibody
- 5388 RXRα Antibody
- 6448 SignalSilence® NRBF-2 siRNA I
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.
