Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

LASP1 Antibody #8636

Applications Reactivity Sensitivity MW (kDa) Source
W H (Mk) Endogenous 38 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LASP1 Antibody detects endogeneous levels of total LASP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala191 of human LASP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LASP1 Antibody.

Background

LASP1 is a cytoskeletal scaffold protein belonging to the LIM protein subfamily (1,2). LASP1 consists of an N-terminal LIM domain, followed by two nebulin repeats, and a C-terminal SH3 domain (1,3). The nebulin repeats interact with actin, while the SH3 domain interacts with palladin (4,5), suggesting LASP1 functions as an actin-binding protein, possibly in cytoskeletal organization. LASP1 has been shown to localize to focal adhesions, lamellipodia, and membrane ruffles (6-8) and might be involved in membrane migration. Overexpression of LASP1 has been associated with metastatic cancers, such as breast and ovarian cancer (2). In these cases, membrane, cytoplasmic, and nuclear localization of LASP1 in the tumor cell has been reported, suggesting LASP1 involvement in membrane and nuclear signaling (9,10).

  1. Tomasetto, C. et al. (1995) FEBS Lett 373, 245-9.
  2. Grunewald, T.G. and Butt, E. (2008) Mol Cancer 7, 31.
  3. Schreiber, V. et al. (1998) Mol Med 4, 675-87.
  4. Chew, C.S. et al. (2002) J Cell Sci 115, 4787-99.
  5. Rachlin, A.S. and Otey, C.A. (2006) J Cell Sci 119, 995-1004.
  6. Chew, C.S. et al. (2000) J Cell Sci 113 ( Pt 11), 2035-45.
  7. Lin, Y.H. et al. (2004) J Cell Biol 165, 421-32.
  8. Grunewald, T.G. et al. (2007) Br J Cancer 96, 296-305.
  9. Frietsch, J.J. et al. (2010) Br J Cancer 102, 1645-53.
  10. Traenka, C. et al. (2010) Cancer Res 70, 8003-14.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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