Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

BATF (D7C5) Rabbit mAb #8638

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H M Endogenous 15 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BATF (D7C5) Rabbit mAb detects endogenous levels of total BATF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BATF protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BATF (D7C5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct encoding full-length human BATF (hBATF; +), using BATF (D7C5) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa (blue) and KARPAS-299 (green) cells using BATF (D7C5) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of KARPAS-299 (left) and HeLa (right) cells using BATF (D7C5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

Basic leucine zipper transcriptional factor ATF-like (BATF) is a basic leucine zipper (bZIP) transcription factor and is part of the AP-1/ATF family that forms inhibitory dimers with members of the Jun family (1-3). Expression of BATF is largely restricted with highest levels found in mature T cells, and it is induced in B cells following immune responses including viral infection (1,2). BATF expression is also induced by IL-6 via a Stat3-dependent mechanism (4). BATF plays an important role in the differentiation of immune cell lineages (5-7). Studies of BATF-deficient mice have demonstrated a critical role for BATF in the formation of IL-17-expressing Th17 cells, in part, by regulating the expression of IL-17 (5,6). BATF knockouts are resistant to experimental autoimmune encephalomyelitis (EEA), consistent with the role of Th17 cells in this model for autoimmunity (5). Additional studies have found that BATF is important in generating antibody class switching. BATF is required for the generation of follicular helper T cells (Tfh), by regulating BCL6 and c-Maf (6,7). In B cells, BATF controls the expression of activation-induced cytidine deaminase (AID) and regulates class-switched antibody responses (7). Taken together, these studies suggest that BATF is a key regulator of distinct populations of immune cells.

  1. Dorsey, M.J. et al. (1995) Oncogene 11, 2255-65.
  2. Hasegawa, H. et al. (1996) Biochem Biophys Res Commun 222, 164-70.
  3. Echlin, D.R. et al. (2000) Oncogene 19, 1752-63.
  4. Senga, T. et al. (2002) Oncogene 21, 8186-91.
  5. Schraml, B.U. et al. (2009) Nature 460, 405-9.
  6. Betz, B.C. et al. (2010) J Exp Med 207, 933-42.
  7. Ise, W. et al. (2011) Nat Immunol 12, 536-43.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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