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AMPA Receptor Antibody Sampler Kit #8652

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Isotype
AMPA Receptor (GluR 2/3/4) Antibody #2460	40 µl	W	H M R	100	Rabbit
AMPA Receptor (GluR 2) (D39F2) Rabbit mAb #5306	40 µl	W	H M R	100	Rabbit IgG
AMPA Receptor (GluR 3) (D47E3) Rabbit mAb #4676	40 µl	W IP	H M R	100	Rabbit IgG
AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb #8070	40 µl	W IP IF-F	H M R	100	Rabbit IgG
Phospho-AMPA Receptor (GluR 1) (Ser845) (D10G5) Rabbit mAb #8084	40 µl	W IP	H M R	100	Rabbit IgG
Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody #3921	40 µl	W	R (H) (M)	100	Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074	100 µl				Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Phospho-AMPA Receptor (GluR 1) (Ser845) (D10G5) Rabbit mAb recognizes endogenous levels of AMPA Receptor (GluR 1) protein only when phosphorylated at Ser845. While the literature refers to this residue as Ser845, it is Ser863 in the UniProt sequence P42261. Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody detects endogenous levels of GluR 2 protein only when phosphorylated at Tyr869, Tyr873, or Tyr876. It may also detect GluR 3 when phosphorylated at the conserved Tyr880, Tyr884, or Tyr887. These residues are not conserved in GluR 1 or GluR 4. AMPA Receptor (GluR 2) (D39F2) Rabbit mAb detects endogenous levels of total GluR 2 protein. The antibody is not predicted to recognize other AMPA receptor subunits (e.g. GluR 1, GluR 3, or GluR 4) based on sequence homology of the antigen. AMPA Receptor (GluR 3) (D47E3) Rabbit mAb detects endogenous levels of total GluR 3 protein. The antibody is not predicted to detect other AMPA receptor subunits (e.g. GluR 1, GluR 2, or GluR 4) based on sequence homology of the antigen. AMPA Receptor (GluR 4) (D41A11) XP^® Rabbit mAb detects endogenous levels of total GluR 4 protein. AMPA Receptor (GluR 2/3/4) Antibody detects endogenous levels of total GluR 2/3/4 protein. It may also detect GluR 1.

Description

The AMPA Receptor Antibody Sampler Kit provides an economical means of evaluating the four subunits of AMPARs. The kit contains enough primary and secondary antibodies to perform four western blot experiments with each antibody.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser720 of human GluR 2 protein or a synthetic phosphopeptide corresponding to residues surrounding Tyr869, Tyr873, and Tyr876 of human GluR 2 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GluR 2 protein, surrounding Pro590 of human GluR 3 protein, surrounding Gln890 of human GluR 4 protein, or surrounding Ser845 of human AMPA Receptor (GluR 1) protein.

Background

AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2-containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).

1. Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77.
2. Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

2460:

Western Blotting

3921:

Western Blotting

4676:

Immunoprecipitation, Western Blotting

5306:

Western Blotting

8070:

Immunofluorescence, Immunoprecipitation, Western Blotting

8084:

Immunoprecipitation, Western Blotting

Companion Products

• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7727 Biotinylated Protein Ladder Detection Pack
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9998 BSA
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
• 9999 Nonfat Dry Milk

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For Research Use Only. Not For Use In Diagnostic Procedures.