Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Organelle Localization IF Antibody Sampler Kit #8653

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
AIF (D39D2) XP® Rabbit mAb #5318 40 µl W IP IF-IC H M R Mk (B) (Dg) 67 Rabbit IgG
EEA1 (C45B10) Rabbit mAb #3288 40 µl W IP IF-IC H M R 170 Rabbit IgG
LAMP1 (D2D11) XP® Rabbit mAb #9091 40 µl W IP IF-IC H Mk 42 (non-glycosylated), 90-120 (glycosylated) Rabbit IgG
PDI (C81H6) Rabbit mAb #3501 40 µl W IHC-P IF-IC H M R Mk 57 Rabbit
RCAS1 (D2B6N) XP® Rabbit mAb #12290 40 µl W IP IF-IC F H M R (Mk) 32 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the Organelle Localization IF Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.

Confocal immunofluorescent analysis of MCF7 cells, untreated (upper) or treated with Brefeldin A #9972 (5 μg/ml, 1 hr; lower), using RCAS1 (D2B6N) XP® Rabbit mAb #12290 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb #3288 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb #3501 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Confocal immunofluorescent analysis of HeLa cells using AIF (D39D2) XP® Rabbit mAb #5318 (green), showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos #9082 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb #9091 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Description

The Organelle Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immunocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four western blots with each antibody.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein, or with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein, residues surrounding Ser70 of human EEA1 protein, the sequence of human PDI protein, or residues surrounding Gly147 of human RCAS1 protein.

Background

Knowledge of the subcellular location of a protein may reveal its potential role in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

* Product-specific protocol.

Companion Products

Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patents No. 5,675,063) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

Products