Cell Signaling Technology

Product Pathways - Protein Stability

Phospho-SGTA (Ser305) (D23E10) Rabbit mAb #8664

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 34 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-SGTA (Ser305) (D23E10) Rabbit mAb recognizes endogenous levels of SGTA protein only when phosphorylated at Ser305.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser305 of human SGTA protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with λ phosphatase (+), using Phospho-SGTA (Ser305) (D23E10) Rabbit mAb (upper) and SGTA Antibody #3349 (lower).

Background

SGTA, small glutamine-rich tetratricopeptide repeat-containing protein A, is an ubiquitously expressed co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity (1,2). SGTA is a 34 kDa protein that is rich in glutamine residues at its C terminus and contains three tandemly repeated TPR motifs (3). The TPR domain of SGTA shows sequence similarity to the TPR domains of Hop, CHIP, and TOM70 (4). The TPR domain of SGTA also interacts with HSP90 and was recently found to be a pro-apoptotic factor (5,6).

Phosphorylation of SGTA at Ser305 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST™ LC-MS/MS platform for phosphorylation site discovery (7). Site-specific mutation analysis indicated that phosphorylation at Ser305 is essential for PDGFR α stabilization and PDGFR α-dependent cancer cell survival (7).

  1. Liu, F.H. et al. (1999) J Biol Chem 274, 34425-32.
  2. Tobaben, S. et al. (2003) J Biol Chem 278, 38376-83.
  3. Cziepluch, C. et al. (1998) J Virol 72, 4149-56.
  4. Scheufler, C. et al. (2000) Cell 101, 199-210.
  5. Liou, S.T. and Wang, C. (2005) Arch Biochem Biophys 435, 253-63.
  6. Yin, H. et al. (2006) Biochem Biophys Res Commun 343, 1153-8.
  7. Moritz, A. et al. (2010) Sci Signal 3, ra64.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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