Product Pathways - PI3K / Akt Signaling
Rag and LAMTOR Antibody Sampler Kit #8665
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb #8975||40 µl||W IP IHC-P IF-IC||H M R Mk||18||Rabbit IgG|
|LAMTOR2/ROBLD3 (D7C10) Rabbit mAb #8145||40 µl||W IP||H M R Mk||14||Rabbit IgG|
|LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb #8168||40 µl||W||H M R Mk||14||Rabbit IgG|
|RagA (D8B5) Rabbit mAb #4357||40 µl||W IP||H M R Mk||30||Rabbit IgG|
|RagB Antibody #3830||40 µl||W||H||40||Rabbit|
|RagC (D8H5) Rabbit mAb #9480||40 µl||W IP IF-IC F||H M R Mk||50||Rabbit IgG|
|RagD Antibody #4470||40 µl||W||H||50||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb, LAMTOR2/ROBLD3 (D7C10) Rabbit mAb, and LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb recognize endogenous levels of each specific protein. In addition, each antibody may cross-react with other LAMTOR proteins. RagA (D8B5) Rabbit mAb recognizes endogenous levels of total RagA protein. This antibody may also recognize endogenous levels of total RagB protein. RagB Antibody recognizes endogenous levels of total RagB protein. RagC (D8H5) Rabbit mAb recognizes endogenous levels of total RagC protein. RagD Antibody recognizes endogenous levels of total RagD protein.
Western blot analysis of extracts from various cell lines using RagB Antibody #3830.
Western blot analysis of extracts from mIMCD3, KNRK, and COS-7 cells using RagA (D8B5) Rabbit mAb #4357.
Western blot analysis of extracts from various cell lines using LAMTOR2/ROBLD3 (D7C10) Rabbit mAb #8145.
Western blot analysis of extracts from various cell lines using LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb #8168.
Western blot analysis of extracts from various cell lines using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb #8975.
The Rag and LAMTOR Antibody Sampler Kit is an economical means of detecting various Rag and LAMTOR proteins implicated within mTOR complex signaling. The kit contains enough primary and secondary antibody to perform four western blots with each antibody.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala71 of human LAMTOR1/C11orf59 protein, residues surrounding Leu31 of human LAMTOR2/ROBLD3 protein, or residues surrounding Val16 of human LAMTOR3/MAPKSP1 protein. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RagA protein or residues in the amino terminus of human RagC protein. Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RagB or RagD proteins. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
The mTORC kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). According to the research literature, RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered to be a viable target for further diagnostic and therapeutic study (5). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit four Rag GTPases to the surface of lysosomes (6).
- Sancak, Y. et al. (2008) Science 320, 1496-501.
- Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
- Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
- Wullschleger, S. et al. (2006) Cell 124, 471-84.
- de Wit, N.J. et al. (2005) Br J Cancer 92, 2249-61.
- Sancak, Y. et al. (2010) Cell 141, 290-303.
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For Research Use Only. Not For Use In Diagnostic Procedures.