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Product last modified at: 2026-03-20T08:02:27.328Z
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SignalKine Human IL-4 Sandwich ELISA Kit #8669

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  • ELISA+

Inquiry Info. # 8669

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    Product Specifications

    REACTIVITY H
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    SignalKine™ Human IL-4 Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human IL-4 (hIL-4) in multiple matrices. Unknown samples being tested for hIL-4 and hIL-4 standards are added to low volume microwells, where the hIL4 is captured by the coated hIL-4 Rabbit mAb. Following a washing step, a biotinylated hIL-4 Detection Rabbit mAb is added to detect the captured hIL-4. HRP-linked Streptavidin is then used for detection of the biotinylated hIL-4 Detection Rabbit mAb. HRP substrate, TMB, is added for color development. The magnitude of absorbance for this developed color is proportional to the quantity of hIL-4 in the sample.
    SignalKine™ Human IL-4 Sandwich ELISA Kit detects hIL-4 in multiple matrices that can be quantified by generating a standard curve with the recombinant hIL-4 protein standard provided. The hIL-4 standard range is from 15.6 to 1000 pg/ ml. Samples containing higher levels of hIL-4 can be diluted to fit into the standards range.

    Specificity / Sensitivity


    Species Reactivity:

    Human

    Background

    Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).

    Alternate Names

    B cell growth factor 1; B cell stimulatory factor 1; B_cell stimulatory factor 1; B-cell stimulatory factor 1; BCGF-1; BCGF1; Binetrakin; BSF-1; BSF1; IL-4; IL4; interleukin 4; interleukin 4 variant 2; Interleukin-4; Lymphocyte stimulatory factor 1; Pitrakinra

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