Cell Signaling Technology

Product Pathways - Vesicle Trafficking

Importin β1 Antibody #8673

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 97 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Importin β1 Antibody detects endogeneous levels of total importin β1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro307 of human importin β1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Importin β1 Antibody.

Background

Importins belong to the karyopherin family of nuclear transport proteins (1) and are divided into two subgroups: importin α and importin β. Importins mainly function in nuclear protein import and export (2,3). Importin β1 (also known as karyopherin β1, Kpnβ1, Kpnb1, or p97) plays a key role in the nuclear import process (1-3). Nuclear import via importin β1 association with adaptor importin α (also known as karyopherin α, or Kpnα) is an essential component of the classical nuclear localization signal (NLS) pathway (4). Importin α directly recognizes the NLS present in the cargo taget, prompting complex formation with importin β1. The cargo:importin α:importin β1 complex is transported across the nuclear pore complex (NPC) into the nucleus, where it is dissociated by the binding of RanGTP (1-4). Nuclear import directly via importin β1 can also occur by importin β1 recognition of the cargo protein, bypassing importin α involvement. In both cases, the importin β1/target protein interaction is mediated through the binding of importin β1 HEAT repeats with the target protein sequences (either the cargo protein itself or importin α) (5).

  1. Chook, Y.M. and Blobel, G. (2001) Curr Opin Struct Biol 11, 703-15.
  2. Pemberton, L.F. and Paschal, B.M. (2005) Traffic 6, 187-98.
  3. Moroianu, J. (1998) J Cell Biochem 70, 231-9.
  4. Lange, A. et al. (2007) J Biol Chem 282, 5101-5.
  5. Marfori, M. et al. (2011) Biochim Biophys Acta 1813, 1562-77.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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