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Mitochondrial Marker Antibody Sampler Kit #8674

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Isotype
COX IV (3E11) Rabbit mAb #4850	40 µl	W IP IHC-P IHC-F IF-IC F	H R Mk Z B Pg	17	Rabbit IgG
Cytochrome c (136F3) Rabbit mAb #4280	40 µl	W IHC-P	H M R Mk	14	Rabbit IgG
HSP60 (D307) Antibody #4870	40 µl	W IF-IC F	H M R Mk Dm	60	Rabbit
PHB1 Antibody #2426	40 µl	W IP	H M R Mk	32	Rabbit
Pyruvate Dehydrogenase (C54G1) Rabbit mAb #3205	40 µl	W IHC-P	H M R Mk	43	Rabbit IgG
SDHA Antibody #5839	40 µl	W IF-IC	H M R Mk	70	Rabbit
SOD1 (71G8) Mouse mAb #4266	40 µl	W IP	H	18	Mouse IgG1
VDAC (D73D12) Rabbit mAb #4661	40 µl	W	H M R Mk	32	Rabbit IgG
Anti-mouse IgG, HRP-linked Antibody #7076	100 µl				Horse
Anti-rabbit IgG, HRP-linked Antibody #7074	100 µl				Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dm=D. melanogaster  Z=Zebrafish  B=Bovine  Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the Mitochondrial Marker Antibody Sampler Kit recognizes endogenous levels of total respective protein. SOD1 (71G8) Mouse mAb does not cross-react with other related proteins. HSP60 (D307) Antibody does not cross-react with other HSPs.

Description

The Mitochondrial Marker Antibody Sampler Kit provides an economical means to evaluate relevant mitochondial proteins. This kit contains enough primary antibody to perform four western blots per primary.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV protein, residues 67-78 of human cytochrome c protein, the sequence of human pyruvate dehydrogenase protein, or residues near the amino terminus of human VDAC-1 protein. Mouse monoclonal antibody is produced by immunizing animals with full-length recombinant human SOD1 protein.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp307 of human HSP60 protein, residues surrounding Ser252 of human PHB1 protein, or residues near the carboxy terminus of human SDHA protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

The Mitochondrial Marker Antibody Sampler Kit contains a variety of antibodies directed against established mitochondrial proteins. Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1). Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to the mitochondrial intermembrane space (2). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (3). In the mitochondria, prohibitins (PHB1) mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (4). In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (5). Succinate dehydrogenase (SDH), also known as Complex II or succinate quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (6). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space (7). Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (8).

1. Ostermeier, C. et al. (1996) Curr Opin Struct Biol 6, 460-6.
2. Schägger, H. (2002) Biochim Biophys Acta 1555, 154-9.
3. Jindal, S. et al. (1989) Mol Cell Biol 9, 2279-83.
4. Tatsuta, T. et al. (2005) Mol Biol Cell 16, 248-59.
5. Strumiło, S. (2005) Acta Biochim Pol 52, 759-64.
6. Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31.
7. Sherman, L. et al. (1983) Proc Natl Acad Sci U S A 80, 5465-9.
8. Craigen, W.J. and Graham, B.H. (2008) J Bioenerg Biomembr 40, 207-12.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

2426:

Immunofluorescence, Immunoprecipitation, Western Blotting

3205:

IHC / Paraffin, Western Blotting

4266:

Immunoprecipitation, Western Blotting

4280:

IHC / Paraffin, Western Blotting

4661:

Western Blotting

4850:

Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence*, Immunoprecipitation, Western Blotting

4870:

Flow, Immunofluorescence, Western Blotting

5839:

Immunofluorescence, Western Blotting

* Product-specific protocol.

Companion Products

• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9998 BSA
• 9999 Nonfat Dry Milk
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
• 7722 Blue Loading Buffer Pack
• 7723 Red Loading Buffer Pack
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7075 Anti-biotin, HRP-linked Antibody

Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc. 

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For Research Use Only. Not For Use In Diagnostic Procedures.