Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Oncogene and Tumor Suppressor Antibody Sampler Kit #8678

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
E-Cadherin (24E10) Rabbit mAb #3195 40 µl W IHC-P IHC-F IF-IC F H M (B) (Dg) (Pg) 135 Rabbit IgG
p53 (7F5) Rabbit mAb #2527 40 µl W IHC-P IF-IC F ChIP H Mk 53 Rabbit IgG
Stathmin Antibody #3352 40 µl W IHC-P H M R Mk 19 Rabbit
BRCA1 Antibody #9010 40 µl W IP H 220 Rabbit
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 40 µl W IP IHC-P IHC-F IF-IC F H M R Hm Mk Dm Z B (C) (X) (Dg) (Pg) 60 Rabbit IgG
PTEN (138G6) Rabbit mAb #9559 40 µl W IP IHC-P H M R Mk 54 Rabbit IgG
Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb #5587 40 µl W H (Mk) 66 Rabbit IgG
HER2/ErbB2 (D8F12) XP® Rabbit mAb #4290 40 µl W IHC-P H (M) (R) 185 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry  ChIP=Chromatin IP
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Dm=D. melanogaster  X=Xenopus  Z=Zebrafish  B=Bovine  Dg=Dog  Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb recognizes endogenous levels of Akt only when phosphorylated at Ser473. BRCA1 Antibody recognizes endogenous levels of total BRCA1 protein. The antibody detects BRAC1 nuclear isoforms 1, 2, and 4, but not BRAC1 cytoplasmic isoforms 3 and 5, and does not detect BRCA2. E-Cadherin (24E10) Rabbit mAb recognizes endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb recognizes endogeneous levels of ERα only when phosphorylated at Ser167. The antibody cross reacts with a nonspecific band at around 77 kDa. HER2/ErbB2 (D8F12) XP® Rabbit mAb recognizes endogenous levels of total HER2/ErbB2 protein. p53 (7F5) Rabbit mAb detects endogenous levels of total p53 protein. The antibody binding has been mapped to the amino terminus region of human p53 protein. PTEN (138G6) Rabbit mAb recognizes endogenous levels of total PTEN protein. Stathmin Antibody recognizes endogenous levels of total stathmin protein. The antibody does not cross-react with related proteins, such as SCG10, SCLIP, and RB3.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and COS cells using p53 (7F5) Rabbit mAb #2527.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using E-Cadherin (24E10) Rabbit mAb #3195.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Stathmin Antibody #3352.


Western Blotting

Western Blotting

Western blot analysis of extracts from PC-3 cells, untreated (-) or LY294002/wortmannin-treated (+), and NIH/3T3 cells, serum-starved (-) or PDGF-treated (+), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-BR-3 and MCF7 cells using HER2/ErbB2 (D8F12) XP® Rabbit mAb #4290.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with Estrodiol/EGF (100 nM each, together for 30 min; +) followed by calf intestinal phosphatase (CIP; +), using Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb #5587 (upper) or an estrogen receptor α mouse mAb (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) and UV-treated (50 mJ/cm2, 30 min; +), using BRCA1 Antibody #9010.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain or various cell lines using PTEN (138G6) Rabbit mAb #9559.

Description

The Oncogenes and Tumor Suppressor Antibody Sampler Kit offers an economical means of investigating proteins commonly involved in the biological pathways behind oncogenesis, tumor metastasis, and cancer pathology. The kit contains enough primary and secondary antibody to perform four western blot experiments with each antibody.

Source / Purification

Phospho-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt protein or Ser167 of human estrogen receptor α protein. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein, residues near the amino terminus of human HER2/ErbB2 protein, the carboxy terminus of human PTEN protein, or with a full-length human p53 fusion protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human BRCA1 protein or residues surrounding Ser38 of human stathmin protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Oncogenesis is a multistep process leading to sequential alterations in several oncogenes, tumor-suppressor genes, and microRNA genes (1,2). These alterations often disrupt the expression, function, and/or activity of proteins regulating cell growth and programmed cell death. Many of the molecular mechanisms and biological pathways driving oncogenesis and cancer pathology have been identified. The signal transduction pathways regulating apoptosis, cell-cycle progression, cell adhesion, cell migration, and DNA damage responses are often disrupted. HER2/ErbB2 (3), E-Cadherin (4), p53 (5,6), Stathmin (7), BRCA1 (8,9), Akt (10), PTEN (11), and Estrogen Receptor α (12) function in many of these pathways.

  1. Berger, A.H. et al. (2011) Nature 476, 163-9.
  2. Peltomäki, P. (2012) Exp Cell Res 318, 299-310.
  3. Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.
  4. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
  5. Rahman, N. and Stratton, M.R. (1998) Annu Rev Genet 32, 95-121.
  6. Freed-Pastor, W.A. and Prives, C. (2012) Genes Dev 26, 1268-86.
  7. Belletti, B. and Baldassarre, G. (2011) Expert Opin Ther Targets 15, 1249-66.
  8. Gayther, S.A. et al. (1999) Am J Hum Genet 65, 1021-9.
  9. Scully, R. and Livingston, D.M. (2000) Nature 408, 429-32.
  10. Jazirehi, A.R. et al. (2012) Am J Cancer Res 2, 178-91.
  11. Saal, L.H. et al. (2008) Nat Genet 40, 102-7.
  12. Pópulo, H. et al. (2012) Int J Mol Sci 13, 1886-918.

Application References

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Protocols

* Product-specific protocol.

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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