Cell Signaling Technology

Product Pathways - Metabolism

Pancreatic Marker Antibody Sampler Kit #8679

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
α-Amylase Antibody #4017 40 µl W R (H) (M) 58 Rabbit
CD31 (PECAM-1) (89C2) Mouse mAb #3528 40 µl W IP IHC-P IF-IC F H 130 Mouse IgG2b
Keratin 17/19 (D32D9) XP® Rabbit mAb #3984 40 µl W IHC-P H M R Mk 48/41 Rabbit IgG
Pdx1 (D59H3) XP® Rabbit mAb #5679 40 µl W IP IF-IC H R 42 Rabbit IgG
PLA2G1B Antibody #8962 40 µl W H 16 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl Horse

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the Pancreatic Marker Antibody Sampler Kit recognizes endogenous levels of total respective protein. CD31 (PECAM-1) (89C2) Mouse mAb does not cross-react with other related proteins.

Western Blotting

Western Blotting

Western blot analysis of extracts from HUVE cells using CD31 (PECAM-1) (89C2) Mouse mAb #3528.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, A549, and A431 cells using Keratin 17/19 (D32D9) XP® Rabbit mAb #3984. As expected, keratin 17 is detected in HeLa and A431 cells, while keratin 19 is detected in A549 cells.

Western Blotting

Western Blotting

Western blot analysis of extracts from AR42J cells using α-Amylase Antibody #4017.


Western Blotting

Western Blotting

Western blot analysis of extracts from INS-1 cells using Pdx1 (D59H3) XP® Rabbit mAb #5679.

Western Blotting

Western Blotting

Western blot analysis of extracts from human pancreas and kidney tissue using PLA2G1B Antibody #8962 (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).

Description

The Pancreatic Marker Antibody Sampler Kit provides an economical means to evaluate relevant pancreatic proteins. This kit contains enough primary antibody to perform four western blots per primary.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human keratin 17 and human keratin 19, or a synthetic peptide corresponding to residues surrounding Val274 of human Pdx1 protein. Mouse monoclonal antibody is produced by immunizing animals with recombinant human CD31 protein.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human carcinoid α-amylase protein or residues surrounding Ala131 of human PLA2G1B protein.

Background

The Pancreatic Marker Antibody Sampler Kit contains a variety of antibodies directed against established pancreatic proteins. α-Amylase catalyzes the cleavage of 1, 4-α-D-glucosidic bonds in oligosaccharides and polysaccharides (1). The enzyme is normally produced and secreted in salivary glands (salivary α-amylase or AMY1) and pancreas (pancreatic α-amylase or AMY2A) (1). CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells, and modulates cell adhesion, endothelial cell migration, and angiogenesis (2). Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (3,4). The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation, and mature β-cell function (5,6). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (6,7). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (7), where it promotes the expression of genes important for β-cell functions, such as insulin, glucokinase, and Glut2 (8-10). Phospholipase A2 (PLA2) is a superfamily of enzymes that hydrolyze glycero-3-phosphocholines and release fatty acids and lysophospholipids (11). PLA2G1B is a member of this superfamily in the 1B group that is expressed most highly in the pancreatic acinar cells (12). Evidence suggests that PLA2G1B plays a role in the absorption and storage of extra energy as fats are metabolized (11,12).

  1. Tomita, N. et al. (1988) Cancer Res 48, 3292-6.
  2. Newman, P.J. (1997) J Clin Invest 99, 3-8.
  3. Moll, R. et al. (1982) Cell 31, 11-24.
  4. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
  5. Kaneto, H. et al. (2008) Endocr J 55, 235-52.
  6. Habener, J.F. et al. (2005) Endocrinology 146, 1025-34.
  7. Guz, Y. et al. (1995) Development 121, 11-8.
  8. Ohlsson, H. et al. (1993) EMBO J 12, 4251-9.
  9. Watada, H. et al. (1996) Diabetes 45, 1826-31.
  10. Waeber, G. et al. (1996) Mol Endocrinol 10, 1327-34.
  11. Labonté, E.D. et al. (2010) FASEB J 24, 2516-24.
  12. Cash, J.G. et al. (2011) Int J Obes (Lond) 35, 877-81.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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