Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CtBP1 (D2D6) Rabbit mAb #8684

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC H M Mk Endogenous 47 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CtBP1 (D2D6) Rabbit mAb recognizes endogenous levels of total CtBP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CtBP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CtBP1 (D2D6) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CtBP1 (D2D6) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma using CtBP1 (D2D6) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using CtBP1 (D2D6) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293T cells using CtBP1 (D2D6) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

CtBP1 (C-terminal binding protein 1) was first recognized as a cellular factor that interacts with the C-terminal portion of adenovirus E1A, a protein involved in the transcriptional regulation of key cellular genes (1). CtBP1 is able to regulate gene activity through its intrinsic dehydrogenase activity (2,3) and by interacting with Polycomb Group (PcG) proteins during development (4). Along with its homologue, CtBP2, it acts as a transcriptional corepressor of zinc-finger homeodomain factor deltaEF1 to regulate a wide range of cellular processes through transrepression mechanisms (5). Through its direct interaction with PRDM16, CtBP1 has been shown to be involved in brown adipose tissue differentiation by mediating the repression of white fat genes and directing differentiation toward the brown fat gene program (6). CtBP1 also plays a role in lipid metabolic pathways and membrane fission by regulating the fission machinery operating Golgi tubular networks (7). CtBP1 has recently been shown to repress transcription of BRCA1 via a redox regulated mechanism (8). Furthermore, it is thought that downregulation of BRCA1 and E-cadherin in invasive ductal breast carcinoma correlates directly with activation of CtBP1 (9).

  1. Schaeper, U. et al. (1995) Proc Natl Acad Sci USA 92, 10467-71.
  2. Balasubramanian, P. et al. (2003) FEBS Lett 537, 157-60.
  3. Kumar, V. et al. (2002) Mol Cell 10, 857-69.
  4. Sewalt, R.G. et al. (1999) Mol Cell Biol 19, 777-87.
  5. Furusawa, T. et al. (1999) Mol Cell Biol 19, 8581-90.
  6. Kajimura, S. et al. (2008) Genes Dev 22, 1397-409.
  7. Cassens, U. et al. (1999) Transfus Med 9, 311-20.
  8. Deng, Y. et al. (2010) Oncogene 29, 6603-8.
  9. Deng, Y. et al. (2011) Mol Carcinog, Epub ahead of print.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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