Product Pathways - NF-kB Signaling
IL-1β Antibody (Mouse Specific) #8689
|8689S||100 µl (10 western blots)||---||In Stock||---|
|8689||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
IL-1β Antibody (Mouse Specific) recognizes endogenous levels of full length mouse IL-1β protein. This antibody can detect 500pg of mature recombinant mouse IL-1β by western blot.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IL-1β protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from Raw 264.7 cells, untreated or LPS-treated (1 μg/ml), using IL-1β Antibody (Mouse Specific).
Interleukin-1β (IL-1β) is a proinflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP); the complex then binds to IL-1RI (1,2). Signaling occurs through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII, which lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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