Product Pathways - NF-kB Signaling
IL-1β Antibody (Mouse Specific) #8689
PhosphoSitePlus® protein, site, and accession data: IL1B
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M | Endogenous | 31 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 8689:
- Western Blotting
Specificity / Sensitivity
IL-1β Antibody (Mouse Specific) recognizes endogenous levels of full length mouse IL-1β protein. This antibody can detect 500pg of mature recombinant mouse IL-1β by western blot.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IL-1β protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Raw 264.7 cells, untreated or LPS-treated (1 μg/ml), using IL-1β Antibody (Mouse Specific).
Western Blotting
Western blot analysis of recombinant Mouse Interleukin-1β (mIL-1β) #5204 using IL-1β Antibody (Mouse Specific).
Background
Interleukin-1β (IL-1β) is a proinflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP); the complex then binds to IL-1RI (1,2). Signaling occurs through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII, which lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).
- Dinarello, C.A. (1996) Blood 87, 2095-147.
- Allan, S.M. et al. (2005) Nat Rev Immunol 5, 629-40.
- Kramer, F. et al. (2008) Mol Immunol 45, 2678-89.
Application References
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Companion Products
- 5204 Mouse Interleukin-1β (mIL-1β)
- 8900 Human Interleukin-1β (hIL-1β)
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
