Product Pathways - Chromatin Regulation / Epigenetics
Lysine Methyltransferase Antibody Sampler Kit #8694
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|G9a/EHMT2 (C6H3) Rabbit mAb #3306||40 µl||W IF-IC||H M R Mk (B) (Pg) (Hr)||140, 165||Rabbit IgG|
|ESET (C1C12) Rabbit mAb #2196||40 µl||W IP IF-IC||H Mk||180||Rabbit IgG|
|ASH2L (D93F6) XP® Rabbit mAb #5019||40 µl||W IP IF-IC||H M R Mk (Dm)||80, 65||Rabbit IgG|
|RBBP5 Antibody #8037||40 µl||W||H M R Mk (C) (X) (B) (Pg) (Hr)||67||Rabbit|
|SET7/SET9 Antibody #2813||40 µl||W IF-IC||H M R Mk||48||Rabbit|
|SMYD2 (D14H7) Rabbit mAb #9734||40 µl||W IP||H M R Mk (Hm) (B) (Dg) (Hr)||49||Rabbit IgG|
|SUV39H1 (D11B6) Rabbit mAb #8729||40 µl||W IP||H M R Mk (B)||48||Rabbit IgG|
|SET8 (C18B7) Rabbit mAb #2996||40 µl||W IF-IC||H M R Mk (B) (Pg) (Hr)||43, 50||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Dm=D. melanogaster X=Xenopus B=Bovine Dg=Dog Pg=Pig Hr=Horse
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in this kit recognizes only the specific target protein and does not cross-react with other family members.
Western blot analysis of extracts from MCF-7 and 293 cells using ESET (C1C12) Rabbit mAb #2196.
Western blot analysis of extracts from various cell lines using SET7/SET9 Antibody #2813.
Western blot analysis of extracts from HCT116 and RAW cells using SET8 (C18B7) Rabbit mAb #2996.
Western blot analysis of extracts from HeLa and 293 cells using G9a/EHMT2 (C6H3) Rabbit mAb #3306.
Western blot analysis of extracts from various cell lines using ASH2L (D93F6) XP® Rabbit mAb #5019.
Western blot analysis of extracts from various cell lines using RBBP5 Antibody #8037.
Western blot analysis of extracts from various cell lines using SUV39H1 (D11B6) Rabbit mAb #8729.
The Lysine Methyltransferase Antibody Sampler Kit provides a fast and economical means to evaluate endogenous levels of lysine methyltransferases. The kit contains enough primary antibody to perform four western blot experiments per primary antibody.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ASH2L protein, the carboxy terminus of human ESET protein, the carboxy terminus of human G9a/EHMT2 protein, human SET8 protein, residues surrounding Val414 of human SMYD2 protein, or residues surrounding Asp380 of human SUV39H1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val407 of human RBBP5 protein or the human SET7/SET9 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
SET domain-containing proteins are potential histone methyltransferases (HMTases), which are classified into subgroups by their putative substrate specificities. Histone H3 Lys9 (H3-K9) methyltransferase group genes include Suv39h1, Suv39h2, G9a, G9a related protein (GLP) and SETDB1/ESET (1). The H3-K9 methylation mark plays an important role as a binding site for the chromo-containing protein, resulting in chromatin compaction and heterochromatin generation (2). Histone H3-K4 methylation is exclusively associated with actively transcribed genes (2). The first H3-K4 methylase complex, COMPASS, was identified in the yeast S. cerevisiae and consists of Set1/KMT2 and seven other polypeptides, Cps60-Cps15 (2). Set1/KMT2 functions within COMPASS and is capable of mono-, di-, and trimethylating H3-K4 (2). There are several Set1 related proteins in mammals including WDR5, RBBP5, ASH2L, CXXC1, and DPY30 (2,3). SET7/SET9 is a member of the SET domain-containing family that can specifically methylate H3-K4, Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (4-6). SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a single-subunit enzyme that mono-methylates histone H4-K20, preferably on nucleosomal substrates (7-9). SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), functions to repress transcription by interacting with the Sin3A repressor complex and methylating H3-K36 (10). SMYD2 also methylates H3-K4 through interaction with HSP90α, and methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (11,12).
- Tachibana, M. et al. (2005) Genes Dev 19, 815-26.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Nishioka, K. et al. (2002) Genes Dev 16, 479-89.
- Kouskouti, A. et al. (2004) Mol Cell 14, 175-82.
- Chuikov, S. et al. (2004) Nature 432, 353-60.
- Fang, J. et al. (2002) Curr Biol 12, 1086-99.
- Xiao, B. et al. (2005) Genes Dev 19, 1444-54.
- Couture, J.F. et al. (2005) Genes Dev 19, 1455-65.
- Brown, M.A. et al. (2006) Mol Cancer 5, 26.
- Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72.
- Huang, J. et al. (2006) Nature 444, 629-32.
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For Research Use Only. Not For Use In Diagnostic Procedures.