Product Pathways - Chromatin Regulation / Epigenetics
Lysine Methyltransferase Antibody Sampler Kit #8694
|8694S||1 Kit (8 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|G9a/EHMT2 (C6H3) Rabbit mAb #3306||40 µl||W, IF-IC||H, M, R, Mk||B, Pg, Hr||140, 165||Rabbit IgG|
|ESET (C1C12) Rabbit mAb #2196||40 µl||W, IP, IF-IC||H, Mk||180||Rabbit IgG|
|ASH2L (D93F6) XP® Rabbit mAb #5019||40 µl||W, IP, IF-IC||H, M, R, Mk||Dm||80, 65||Rabbit IgG|
|RBBP5 Antibody #8037||40 µl||W||H, M, R, Mk||C, X, B, Pg, Hr||67||Rabbit|
|SET7/SET9 Antibody #2813||40 µl||W, IF-IC||H, M, R, Mk||48||Rabbit|
|SMYD2 (D14H7) Rabbit mAb #9734||40 µl||W, IP||H, M, R, Mk||Hm, B, Dg, Hr||49||Rabbit IgG|
|SUV39H1 (D11B6) Rabbit mAb #8729||40 µl||W, IP||H, M, R, Mk||B||48||Rabbit IgG|
|SET8 (C18B7) Rabbit mAb #2996||40 µl||W, IF-IC||H, M, R, Mk||B, Pg, Hr||43||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Western blot analysis of extracts from MCF-7 and 293 cells using ESET (C1C12) Rabbit mAb #2196.
Western blot analysis of extracts from various cell lines using SET7/SET9 Antibody #2813.
Western blot analysis of extracts from HCT116 and RAW cells using SET8 (C18B7) Rabbit mAb #2996.
Western blot analysis of extracts from HeLa and 293 cells using G9a/EHMT2 (C6H3) Rabbit mAb #3306.
Western blot analysis of extracts from various cell lines using ASH2L (D93F6) XP® Rabbit mAb #5019.
Western blot analysis of extracts from various cell lines using RBBP5 Antibody #8037.
Western blot analysis of extracts from various cell lines using SUV39H1 (D11B6) Rabbit mAb #8729.
The Lysine Methyltransferase Antibody Sampler Kit provides a fast and economical means to evaluate endogenous levels of lysine methyltransferases. The kit contains enough primary antibody to perform four western blot experiments per primary antibody.
Specificity / Sensitivity
Each antibody in this kit recognizes only the specific target protein and does not cross-react with other family members.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ASH2L protein, the carboxy terminus of human ESET protein, the carboxy terminus of human G9a/EHMT2 protein, human SET8 protein, residues surrounding Val414 of human SMYD2 protein, or residues surrounding Asp380 of human SUV39H1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val407 of human RBBP5 protein or the human SET7/SET9 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
SET domain-containing proteins are potential histone methyltransferases (HMTases), which are classified into subgroups by their putative substrate specificities. Histone H3 Lys9 (H3-K9) methyltransferase group genes include Suv39h1, Suv39h2, G9a, G9a related protein (GLP) and SETDB1/ESET (1). The H3-K9 methylation mark plays an important role as a binding site for the chromo-containing protein, resulting in chromatin compaction and heterochromatin generation (2). Histone H3-K4 methylation is exclusively associated with actively transcribed genes (2). The first H3-K4 methylase complex, COMPASS, was identified in the yeast S. cerevisiae and consists of Set1/KMT2 and seven other polypeptides, Cps60-Cps15 (2). Set1/KMT2 functions within COMPASS and is capable of mono-, di-, and trimethylating H3-K4 (2). There are several Set1 related proteins in mammals including WDR5, RBBP5, ASH2L, CXXC1, and DPY30 (2,3). SET7/SET9 is a member of the SET domain-containing family that can specifically methylate H3-K4, Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (4-6). SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a single-subunit enzyme that mono-methylates histone H4-K20, preferably on nucleosomal substrates (7-9). SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), functions to repress transcription by interacting with the Sin3A repressor complex and methylating H3-K36 (10). SMYD2 also methylates H3-K4 through interaction with HSP90α, and methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (11,12).
- Tachibana, M. et al. (2005) Genes Dev 19, 815-26.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Nishioka, K. et al. (2002) Genes Dev 16, 479-89.
- Kouskouti, A. et al. (2004) Mol Cell 14, 175-82.
- Chuikov, S. et al. (2004) Nature 432, 353-60.
- Fang, J. et al. (2002) Curr Biol 12, 1086-99.
- Xiao, B. et al. (2005) Genes Dev 19, 1444-54.
- Couture, J.F. et al. (2005) Genes Dev 19, 1455-65.
- Brown, M.A. et al. (2006) Mol Cancer 5, 26.
- Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72.
- Huang, J. et al. (2006) Nature 444, 629-32.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.