Product Pathways - Tyrosine Kinase / Adaptors

pathway
application references
companion products
datasheet PDF
MSDS PDF
protocols

Angiogenesis Antibody Sampler Kit #8696

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Isotype
Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478	40 µl	W IHC-P IF-IC	H M	230	Rabbit IgG
Phospho-Akt (Ser473) (D9E) XP^® Rabbit mAb #4060	40 µl	W IP IHC-P IHC-F IF-IC F	H M R Hm Mk Dm Z B (C) (X) (Dg) (Pg)	60	Rabbit IgG
Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943	40 µl	W IP	H M R	60	Rabbit IgG
Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556	40 µl	W IP	H (M) (R) (Mk)	125	Rabbit IgG
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP^® Rabbit mAb #4511	40 µl	W IP IHC-P IF-IC F	H M R Mk Mi Pg Sc (Hm) (C) (Z) (B)	43	Rabbit IgG
Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb #8713	40 µl	W IP IHC-P IF-IC F	H M Mk (R)	150	Rabbit IgG
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP^® Rabbit mAb #4370	40 µl	W IP IHC-P IF-IC F	H M R Hm Mk Mi Dm Z B Dg Pg Sc (C) (Ce)	44, 42	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074	100 µl				Goat

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Mi=Mink Dm=D. melanogaster X=Xenopus Z=Zebrafish B=Bovine Dg=Dog Pg=Pig Sc=S. cerevisiae Ce=C. elegans
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the Angiogenesis Antibody Sampler Kit recognizes the phosphorylated form of its specific target. Phospho-FAK (Tyr397) (D20B1) Rabbit mAb may cross-react with overexpressed tyrosine phosphorylated proteins such as EGFR. Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb may cross-react with other Src family members or overexpressed phosphorylated RTKs. Phospho-VEGFR2 (Tyr1175) (19A10) Rabbit mAb may cross-react with VEGFR1.

Western Blotting

Western blot analysis of extracts from HUVE cells, untreated (-) or VEGF-treated (50 ng/ml, 2 min; +), using Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (upper) or VEGF Receptor 2 (55B11) Rabbit mAb #2479 (lower).

Western Blotting

Western blot analysis of extracts from PC-3 cells, untreated (-) or treated with LY294002 #9901 and wortmannin #9951 (+), and NIH/3T3 cells, serum-starved (-) or PDGF-treated (+), using Phospho-Akt (Ser473) (D9E) XP^® Rabbit mAb #4060 (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Western Blotting

Western blot analysis of extracts from 293, NIH/3T3, and C6 cells, treated with λ phosphatase or TPA #4174 as indicated, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP^® Rabbit mAb #4370 (upper), or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).

Western Blotting

Western blot analysis of extracts from COS and 293 cells, untreated (-) or UV-treated (+), using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP^® Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).

Western Blotting

Western blot analysis of extracts NIH/3T3 cells, serum-starved (-) or treated with hPDGF-BB #8912 (100 ng/ml, 15 min; +), using Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943 (upper) or Src (36D10) Rabbit mAb #2109 (lower).

Western Blotting

Western blot analysis of extracts from A549 cells, untreated (-) or treated with calf intestinal phosphatase (CIP; +), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556 (upper) or FAK Antibody #3285 (lower).

Western Blotting

Western blot analysis of extracts from serum-starved A-431 and A549 cells, untreated (-) or treated with hEGF #8916 (100 ng/ml, 15 min; +), or serum-starved NIH/3T3 cells, untreated (-) or treated with hPDGF-BB #8912 (50 ng/ml, 15 min; +), using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb #8713 (upper) or PLCγ1 (D9H10) XP^® Rabbit mAb #5690 (lower).

Description

The Angiogenesis Antibody Sampler Kit provides an economical means to investigate the angiogenic pathway downstream of VEGFR2. The kit contains enough primary antibody to perform four western blots per primary antibody.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser473 of human Akt protein, Tyr397 of human FAK protein, Thr180/Tyr182 of human p38 MAPK protein, Thr202/Tyr204 of human p44 MAPK protein, Ser1248 of human PLCγ1 protein, Tyr416 of human Src protein, and Tyr1175 of human VEGFR2 protein.

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Signaling from VEGFR2 is necessary for angiogenesis in vivo (2-4). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (5). Phosphorylation of VEGFR2 at Tyr1212 provides a docking site for GRB2 binding and phosphorylation at Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ (1,5,6). Activation of VEGFR2 during angiogenesis leads to signaling through multiple downstream kinase pathways including Akt, Src, FAK, p38, and Erk1/2 (2,7).

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

* Product-specific protocol.

Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.