Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

PRK2 (D10A10) Rabbit mAb #8697

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk B Pg Endogenous 140 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey  B=Bovine  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PRK2 (D10A10) Rabbit mAb recognizes endogenous levels of total PRK2 protein. This antibody does not cross-react with PRK1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu633 of human PRK2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PRK2 (D10A10) Rabbit mAb.

Background

The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).

  1. Mukai, H. et al. (1994) Biochem. Biophys. Res. Commun. 199, 897-904.
  2. Morrice, N.A. et al. (1994) J. Biol. Chem. 269, 20040-20046.
  3. Palmer, R.H. et al. (1994) FEBS Lett. 356, 5-8.
  4. Watanabe, G. et al. (1996) Science 271, 645-648.
  5. Amano, M. et al. (1996) Science 271, 648-650.
  6. Vincent, S. and Settleman, J. (1997) Mol. Cell. Biol. 17, 2247-2256.
  7. Morrice, N.A. et al. (1994) FEBS Lett. 351, 171-175.
  8. Palmer, R.H. et al. (1995) J. Biol. Chem. 270, 22412-22416.
  9. Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-70.
  10. Dong, L.Q. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5089-94.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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