Cell Signaling Technology

Product Pathways - Neuroscience

Rhodopsin Antibody #8710

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Hm) (Mk) Endogenous 37, 75 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rhodopsin Antibody recognizes endogenous levels of total rhodopsin protein. This antibody may also detect rhodopsin protein at 55 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro7 of human rhodopsin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat eye using Rhodopsin Antibody.

Background

Rhodopsin is the photoreceptor in the retinal rods. It is activated by photons, transduces visual information through its cognate G protein, transducin, and is inactivated by arrestin binding (1). Using atomic-force microscopy, rhodopsin was found to be arranged into paracrystalline arrays of dimers in mouse disc membranes (2). Rhodopsin is considered to be the prototype of G protein-coupled receptors (GPCRs), and is the first GPCR for which a crystal structure was solved (3). Research studies have linked mutations in the gene encoding rhodopsin to retinitis pigmentosa (4,5), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness (6).

  1. Arshavsky, V.Y. and Burns, M.E. (2012) J Biol Chem 287, 1620-6.
  2. Fotiadis, D. et al. (2003) Nature 421, 127-8.
  3. Palczewski, K. et al. (2000) Science 289, 739-45.
  4. Rivolta, C. et al. (2002) Hum Mol Genet 11, 1219-27.
  5. Wilson, J.H. and Wensel, T.G. (2003) Mol Neurobiol 28, 149-58.
  6. Hartong, D.T. et al. (2006) Lancet 368, 1795-809.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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