Cell Signaling Technology

Product Pathways - Apoptosis

Apaf-1 (D7G4) Rabbit mAb #8723

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R (Mk) (B) (Pg) Endogenous 135 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Apaf-1 (D7G4) Rabbit mAb recognizes endogenous levels of total Apaf-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Apaf-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Apaf-1 siRNA I (+), using Apaf-1 (D7G4) Rabbit mAb #8723 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Apaf-1 (D7G4) Rabbit mAb confirms silencing of Apaf-1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Apaf-1 (D7G4) Rabbit mAb.

Background

Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3 (2,3). The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat (4). Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli (5,6).

  1. Zou, H. et al. (1997) Cell 90, 405-13.
  2. Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
  3. Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
  4. Benedict, M.A. et al. (2000) J Biol Chem 275, 8461-8.
  5. Cecconi, F. et al. (1998) Cell 94, 727-37.
  6. Yoshida, H. et al. (1998) Cell 94, 739-50.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products