Product Pathways - Apoptosis
Apaf-1 (D7G4) Rabbit mAb #8723
|8723S||100 µl (10 western blots)||---||In Stock||---|
|8723||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat||Endogenous||135||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Monkey, Bovine, Pig.
Specificity / Sensitivity
Apaf-1 (D7G4) Rabbit mAb recognizes endogenous levels of total Apaf-1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Apaf-1 protein.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Apaf-1 siRNA I (+), using Apaf-1 (D7G4) Rabbit mAb #8723 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Apaf-1 (D7G4) Rabbit mAb confirms silencing of Apaf-1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.
Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3 (2,3). The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat (4). Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli (5,6).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 8969 Apaf-1 (D5C3) Rabbit mAb
- 5088 Apaf-1 (R205) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.