Product Pathways - Companion Products
Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) #8726
| Applications | Reactivity | Isotype |
|---|---|---|
| IP | Rabbit IgG |
Applications Key:
IP=Immunoprecipitation
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 8726:
- Immunoprecipitation
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.
Source / Purification
IP
Immunoprecipitation of HeLa cell lysates using EGF Receptor (D38B1) XP® Rabbit mAb (Magnetic Bead Conjugate) #6937 (lane 1) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) (lane 2). The western blot was probed using EGF Receptor (D38B1) XP® Rabbit mAb #4267.
Description
This Cell Signaling Technology (CST) antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
