Product Pathways - Protein Stability
USP8 Antibody #8728
|8728S||100 µl (10 western blots)||---||In Stock||---|
|8728||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
USP8 Antibody recognizes endogenous levels of total USP8 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro320 of human USP8 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indespensible for cell proliferation and survival (3,4). Indeed, conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases, including EGFR, ErbB3, and c-Met (4). In agreement with these findings, USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR (5,6). Furthermore, phosphorylation of USP8 at Ser680 results in its binding of 14-3-3, catalytic inactivation, and reduced EGFR deubiquitination (7). It appears as though USP8, in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation (8,9).
- Nijman, S.M. et al. (2005) Cell 123, 773-86.
- Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
- Naviglio, S. et al. (1998) EMBO J 17, 3241-50.
- Niendorf, S. et al. (2007) Mol Cell Biol 27, 5029-39.
- Alwan, H.A. and van Leeuwen, J.E. (2007) J Biol Chem 282, 1658-69.
- Mizuno, E. et al. (2005) Mol Biol Cell 16, 5163-74.
- Mizuno, E. et al. (2007) Exp Cell Res 313, 3624-34.
- Row, P.E. et al. (2006) J Biol Chem 281, 12618-24.
- Berlin, I. et al. (2010) J Biol Chem 285, 34909-21.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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