Cell Signaling Technology

Product Pathways - Protein Stability

USP8 Antibody #8728

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 130 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

USP8 Antibody recognizes endogenous levels of total USP8 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro320 of human USP8 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using USP8 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected (+) with a Myc/DDK-tagged cDNA expression construct encoding full-length human USP8, using USP8 Antibody.

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indespensible for cell proliferation and survival (3,4). Indeed, conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases, including EGFR, ErbB3, and c-Met (4). In agreement with these findings, USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR (5,6). Furthermore, phosphorylation of USP8 at Ser680 results in its binding of 14-3-3, catalytic inactivation, and reduced EGFR deubiquitination (7). It appears as though USP8, in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation (8,9).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Naviglio, S. et al. (1998) EMBO J 17, 3241-50.
  4. Niendorf, S. et al. (2007) Mol Cell Biol 27, 5029-39.
  5. Alwan, H.A. and van Leeuwen, J.E. (2007) J Biol Chem 282, 1658-69.
  6. Mizuno, E. et al. (2005) Mol Biol Cell 16, 5163-74.
  7. Mizuno, E. et al. (2007) Exp Cell Res 313, 3624-34.
  8. Row, P.E. et al. (2006) J Biol Chem 281, 12618-24.
  9. Berlin, I. et al. (2010) J Biol Chem 285, 34909-21.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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