Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SUV39H1 (D11B6) Rabbit mAb #8729

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk (B) Endogenous 48 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SUV39H1 (D11B6) Rabbit mAb recognizes endogenous levels of total SUV39H1 protein. This antibody does not cross-react with the SUV39H2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp380 of human SUV39H1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SUV39H1 (D11B6) Rabbit mAb.

Background

Human histone methyltransferase SUV39H1 is the homolog of the dominant Drosophila PEV modifier Su(var)3-9 and is composed of 412 amino acid residues (1). It combines two of the most evolutionarily conserved domains of the "chromatin regulators": the chromo and SET domains (2,3). The 60 amino acid chromo domain represents an ancient histone-like fold that directs heterochromatic localizations. It has been demonstrated that the 130 amino acid SET domain contains the methyltransferase catalytic motif, which cooperates with the adjacent cysteine-rich regions to confer histone methyltransferase activity (1). This enzyme selectively methylates histone H3 on Lys9, which generates a binding site for HP1 proteins, a family of heterochromatic adaptor molecules involved in both gene silencing and supra-nucleosomal chromatin structure (4,5). SUV39H1 histone methyltransferase plays an important role in modification of histone amino termini and regulation of gene expression.

  1. Rea, S. et al. (2000) Nature 406, 593-599.
  2. Koonin, E. V. et al. (1995) Nucleic Acids Res. 23, 4229-4232.
  3. Jenuwein, T. et al. (1998) Cell. Mol. Life Sci. 54, 80-93.
  4. Lachner, M. et al. (2001) Nature 410, 116-120.
  5. Bannister, A. J. et al. (2001) Nature 410, 120-123.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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