Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb #8738

Applications Reactivity Sensitivity Isotype
W All Endogenous Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

Specificity / Sensitivity

Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb recognizes endogenous proteins containing a pS/pTDXE motif, which is a CK2 phosphorylation consensus sequence. This antibody is a useful tool to study CK2 substrates.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the T*DXE motif.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902 (10 nM, 30 min) or Staurosporine #9953 (1 μM, 3 hr), using Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® CK2α siRNA I #6389 (+), using Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb (upper), CK2α Antibody #2656 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

Casein Kinase II (CK2) is a highly conserved, ubiquitously expressed, and constitutively active tetrameric Ser/Thr protein kinase with hundreds of substrates participating in the regulation of a variety of cellular processes including cell cycle progression, apoptosis, transcription, inflammation, and the DNA damage response. Research studies have implicated CK2 in roles related to viral infection, cancer, and other diseases (1-5). CK2 substrates contain multiple acidic residues (Asp and Glu) located downstream of the phosphorylated Ser or Thr residue. The consensus sequence for CK2 substrates is pS/pTD/EXD/E with the most crucial residue at the +3 position followed by the residue at the +1 position (6).

  1. Pinna, L.A. and Allende, J.E. (2009) Cell Mol Life Sci 66, 1795-9.
  2. St-Denis, N.A. and Litchfield, D.W. (2009) Cell Mol Life Sci 66, 1817-29.
  3. Trembley, J.H. et al. (2009) Cell Mol Life Sci 66, 1858-67.
  4. Perez, D.I. et al. (2011) Med Res Rev 31, 924-54.
  5. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
  6. Meggio, F. and Pinna, L.A. (2003) FASEB J 17, 349-68.

Application References

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Companion Products

License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.’s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

For Research Use Only. Not For Use In Diagnostic Procedures.

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