Cell Signaling Technology

Product Pathways - Neuroscience

Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 594 Conjugate) #8743

Applications Reactivity Sensitivity Isotype
IF-F H M R Endogenous Rabbit IgG

Applications Key:  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 594 Conjugate) detects endogenous levels of total Neurofilament-L protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu450 of human Neurofilament-L protein.

IF-F

IF-F

Confocal immunofluorescent analysis of rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 594 Conjugate) (red) and GFAP (GA5) Mouse mAb #3670 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat brain. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Neurofilament-L (C28E10) Rabbit mAb #2837.

Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

  1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
  2. Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
  3. Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
  4. Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.

Application References

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This antibody is available exclusively from Cell Signaling Technology (CST) and is not available from other vendors. This antibody is developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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