Product Pathways - Ca / cAMP / Lipid Signaling
WFS1 Antibody #8749
PhosphoSitePlus® protein, site, and accession data: WFS1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | Mk H | Endogenous | 100 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
WFS1 Antibody recognizes endogenous levels of total WFS1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala43 of human WFS1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using WFS1 Antibody.
Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® WFS1 siRNA I #7044 (+), using WFS1 Antibody (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).
Background
Wolfram syndrome protein (WFS1) is an 890 amino acid protein that contains a cytoplasmic N-terminal domain, followed by nine-transmembrane domains and a luminal C-terminal domain. WFS1 is predominantly localized to the endoplasmic reticulum (ER) (1) and its expression is induced in response to ER stress, partially through transcriptional activation (2,3). Research studies have shown that mutations in the WFS1 gene lead to Wolfram syndrome, an autosomal recessive neurodegenerative disorder defined by young-onset, non-immune, insulin-dependent diabetes mellitus and progressive optic atrophy (4).
- Takeda, K. et al. (2001) Hum Mol Genet 10, 477-84.
- Yamaguchi, S. et al. (2004) Biochem Biophys Res Commun 325, 250-6.
- Ueda, K. et al. (2005) Eur J Endocrinol 153, 167-76.
- Inoue, H. et al. (1998) Nat Genet 20, 143-8.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
