Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Lineage Markers

Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #8750

Applications Reactivity Sensitivity Isotype
IF-IC H (Mk) Endogenous Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) detects endogenous levels of total nanog protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human nanog protein.

IF-IC

IF-IC

Immunofluorescent analysis of NTERA-2 (left) and HeLa (right) cells using Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) (red). Actin filaments were labeled with Alexa Fluor® 488 phalloidin (green).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Nanog (D73G4) XP® Rabbit mAb #4903.

Background

Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).

  1. Kim, J. et al. (2008) Cell 132, 1049-61.
  2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
  3. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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