Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways -

Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 594 Conjugate) #8760

Applications Reactivity Sensitivity Isotype
IF-IC Endogenous Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 594 Conjugate) is not expected to recognize any specific antigen. It functions as an isotype control for rabbit monoclonal antibodies.

Source / Purification

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 594 Conjugate) #7634 (red; left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 594 Conjugate) (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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